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51.
Kusaka T Ueno M Miki T Kanenishi K Nagai Y Huang CL Okamoto Y Ogawa T Onodera M Itoh S Akiguchi I Sakamoto H 《Microscopy research and technique》2007,70(7):648-655
We investigated whether beta-amyloid (Abeta)-like immunoreactivity was seen in the brains of newborn piglets. The immunoreactivity for Abeta(1-42) and Abeta(1-40) proteins, but not Abeta precursor protein, was present in CD68-positive perivascular cells of the hippocampus and in parts of the meninges. It was colocalized with immunoreactivity for receptor for advanced glycation end product and tumor necrosis factor-alpha. The protein with a molecular mass of 27 kDa, which was recognized by the Abeta antibodies, was identified as triosephosphate isomerase (TPI) with sequence homology to Abeta peptides by N-terminal amino acid sequencing, mass fingerprint analysis using matrix-associated laser desorption/ionization mass spectrometry, and Western blotting. Western blotting assay also revealed that detectable expression of Abeta proteins were not seen in the piglet brains. These findings indicate that TPI with sequence homology to Abeta peptides accumulates in perivascular cells of the microglia/macrophage lineage located around arterial vessels of the newborn piglet hippocampus. 相似文献
52.
Yoshihiro Adachi Kensei Tsuchida Takanori Imaki Takeo Yaku 《Electronic Notes in Theoretical Computer Science》2000,30(4):276-288
An intelligible program diagram called Logichart has been developed for Prolog visualization. Its syntax rules and layout rules are formalized in terms of an attribute graph grammar. This grammar is underlain by a context–free graph grammar whose productions are defined to formalize the graph–syntax rules of Logichart diagrams. Semantic rules attached to the productions are defined in such a way that they can extract the layout information needed to display a Logichart diagram as attributes. Our Prolog visualization system, which has been implemented based on the attribute graph grammar, can draw a Logichart diagram for any correct Prolog program (completeness), and any Logichart diagram displayed by the system is always valid for the grammar (soundness). The system can also display the execution of a Prolog program in real time; that is, the Logichart diagram is dynamically updated as the program runs. 相似文献
53.
The basic constructs of SIMPOS (Sequential Inference Machine Programming and Operating System) are explained. SIMPOS is an operating system for a super-personal computer (SIM), based on logic programming language (a modified Prolog, called KL0). Our design principle is simplicity both in concept and structure. The entire system will be constructed using these basic constructs. 相似文献
54.
Ranasinghe PD Satoh H Oshiki M Oshima K Suda W Hattori M Mino T 《Water science and technology》2012,66(10):2155-2161
The diversity of bacterial groups in activated sludge from large- and small-scale wastewater treatment plants was explored by barcoded pyrosequencing of 16S rRNA gene. Activated sludge samples (three small and 17 large scale) were collected from 12 wastewater treatment plants to clarify precise taxonomy and relative abundances. DNA was extracted, and amplified by 4 base barcoded 27f/519r primer set. The 454 Titanium (Roche) pyrosequences were obtained and analyses performed by Quantitative Insight Into Microbial Ecology (QIIME) with around 100,000 reads. Sequence statistics were computed, while constructing a phylogenetic tree and heatmap. Computed results explained total microbial diversity at phylum and class level and resolution was further extended to Operational Taxonomic Unit (OTU) based taxonomic assignment for investigating community distribution based on individual sample. Composition of sequence reads were compared and microbial community structures for large- and small-scale treatment plants were identified as major phyla (Proteobacteria and Bacteroidetes) and classes (Betaproteobacteria and Bacteroidetes). Also, family level breakdowns were explained and differences in family Nitrospiraceae and phylum Actinobacteria found at their species level were also illustrated. Thus, the pyrosequencing method provides high resolution insight into microbial community structures in activated sludge that might have been unnoticed with conventional approaches. 相似文献
55.
A neural oscillator with a double-chain structure is one of the central pattern generator models used to simulate and understand rhythmic movements in living organisms. However, it is difficult to reproduce desired rhythmic signals by tuning an enormous number of parameters of neural oscillators. In this study, we propose an automatic tuning method consisting of two parts. The first involves tuning rules for both the time constants and the amplitude of the oscillatory outputs based on theoretical analyses of the relationship between parameters and outputs of the neural oscillators. The second involves an evolutionary tuning method with a two-step genetic algorithm (GA), consisting of a global GA and a local GA, for tuning parameters such as neural connection weights that have no exact tuning rule. Using numerical experiments, we confirmed that the proposed tuning method could successfully tune all parameters and generate sinusoidal waves. The tuning performance of the proposed method was less affected by factors such as the number of excitatory oscillators or the desired outputs. Furthermore, the proposed method was applied to the parameter-tuning problem of some types of artificial and biological wave reproduction and yielded optimal parameter values that generated complex rhythmic signals in Caenorhabditis elegans without trial and error. 相似文献
56.
Network environments give computer users the option of employing distributed information and services to complete a task. However, gathering information and using services distributed in networks requires knowing exactly what kinds of information and services are required for a task, where they are, and how they can be obtained or utilized. Tracking down the answers to these questions can be difficult, time consuming tasks for users. Mobile agent technology is expected to release them from having to do so. Instead, “intelligent” mobile agents will comprehend the user's requirements, search network nodes autonomously for appropriate information and services, and return with the answers. But several problems must be solved before we can expect agents to perform such actions effectively. We focus on the question of intelligence as a prerequisite for agent functions. What sort of intelligence is expected of agents? We have adopted a model based on the ability to make flexible plans. Specifically, we think mobile agents must be able to: understand user requirements; plan actions that will satisfy the requirements act according to the plan; modify the plan according to actual conditions when they differ from those initially expected; and execute the modified plan. We have implemented these functions in the Plangent system and validated their effectiveness in several example applications. We describe how we combined these planning functions with mobile agent facilities, and show how the agents behave intelligently in an example application of personal travel assistance 相似文献
57.
Reiji Hattori Shuhei Yamada Yoshitomo Masuda Norio Nihei 《Journal of the Society for Information Display》2004,12(1):75-80
Abstract— We have developed new powder materials that exhibit liquid behavior, which can lead to the realization of novel bistable and reflective displays having paper‐white appearance, high contrast, and quick response. Two types of display were demonstrated, one had 160 × 160 array of pixels and the other had 320 × 320 in a 3.1‐in.‐diagonal viewable image size corresponding to 66 and 132 dpi, respectively. These displays were driven by passive‐matrix addressing. The displays showed a reflectivity of more than 41%, a contrast more than 1:10, and a pixel response time of less than 0.2 msec. The seven‐segment display for use in clocks was also demonstrated. 相似文献
58.
Takayoshi Yokoya Rikiya Yoshida Yuki Utsumi Koji Tsubota Hiroyuki Okazaki Takanori Wakita Yoshikazu Mizuguchi Yoshihiko Takano Takayuki Muro Yukako Kato Hiroshi Kumigashira Masaharu Oshima Hisatomo Harima Yoshihiro Aiura Hitoshi Sato Akihiro Ino Hirofumi Namatame Masaki Taniguchi Masaaki Hirai Yuji Muraoka 《Science and Technology of Advanced Materials》2012,13(5)
We have characterized the electronic structure of FeSe1−xTex for various x values using soft x-ray photoemission spectroscopy (SXPES), high-resolution photoemission spectroscopy (HRPES) and inverse photoemission spectroscopy (IPES). The SXPES valence band spectral shape shows that the 2 eV feature in FeSe, which was ascribed to the lower Hubbard band in previous theoretical studies, becomes less prominent with increasing x. HRPES exhibits systematic x dependence of the structure near the Fermi level (EF): its splitting near EF and filling of the pseudogap in FeSe. IPES shows two features, near EF and approximately 6 eV above EF; the former may be related to the Fe 3d states hybridized with chalcogenide p states, while the latter may consist of plane-wave-like and Se d components. In the incident electron energy dependence of IPES, the density of states near EF for FeSe and FeTe has the Fano lineshape characteristic of resonant behavior. These compounds exhibit different resonance profiles, which may reflect the differences in their electronic structures. By combining the PES and IPES data the on-site Coulomb energy was estimated at 3.5 eV for FeSe. 相似文献
59.
Our purpose is to develop the technology for evaluating emotion objectively from the oxygenated hemoglobin within a brain. Nowadays, Japan is an aging society. The elderly people who need care will increase from now on increasingly. In the case of the person requiring the care who lost the function to convey an intention especially, the objective judgment to a physical and mental pain is required. Persons requiring care will also increase in number with the increase in this population. We gave subject stimulus of a comfortable or an uncomfortable sound and measured concentration of the oxygenated hemoglobin of a frontal lobe part by near-infrared spectroscopy. Based on the experimental result, a comfortable state or an uncomfortable state was distinguished by concentration of the oxygenated hemoglobin using the bayesian network. As a result, we were able to estimate the subject’s psychological condition. 相似文献
60.
Ya-Li Wang Zhi-Jun Liu Qiang Liu Jian-Nan Ma Masao Hattori Chao-Mei Ma 《Food Analytical Methods》2014,7(7):1526-1529
Two cyanogenic glycosides (linustatin and neolinustatin) and sucrose were isolated from defatted flaxseed, and their structures were determined by NMR spectral analysis and comparison with existing data. Using secoisolariciresinol diglucoside (SDG) and the cyanogenic glycosides as standards, we developed a method to quantify the three compounds simultaneously by ultrahigh-performance liquid chromatography (UHPLC)-MS in selected reaction monitoring (SRM) mode. Using this tool, flaxseed powder—processed by various methods—was analyzed with regard to the contents of the beneficial compound, SDG, and the toxic compounds, cyanogenic glycosides. The levels of SDG in samples treated with alkaline solution are much higher than in samples without treatment, and in general, the levels of linustatin and neolinustatin in flaxseed powder decreased progressively as heating time is prolonged after treatment with alkaline solutions. 相似文献